Text S1: Sequencing, Alignment and SNP Calls
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چکیده
We used a mixed 454 and Illumina sequencing strategy. Genomic DNA from each House Finch strain was first nebulized and blunt-end ligated with Roche adaptors. Emulsion PCR was performed using the bulk library and these products were pyrosequenced on Roche 454 Gene Sequencer using FLX chemistry, using physical separation of isolates. Genomic DNA from the four newly sequenced poulty strains (Table S1) was sequenced by the Illumina method at the University of Utah Huntsman Cancer Institute.
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تاریخ انتشار 2011